Optimizing a novel method for low intensity ultrasound in chondrogenesis induction

Optimizing a novel method for low intensity ultrasound in chondrogenesis induction

BACKGROUND Hyaline cartilage tissue of joints is susceptible to injuries due to avascularity. Mesenchymal stem cells (MSCs) are used for cartilage tissue engineering. Among MSCs, adipose stem cells (ASCs) are attractive because of accessibility, their large number, and rapid growth. Common in vitro protocols successfully induce chondrogenic differentiation by expression of multiple cartilage-sp...

Optimizing Ultrasonic Intensity for High Intensity Focused Ultrasound Therapy

Low Intensity Ultrasound, Proliferation & Growth Indexes in Adult Stem Cell

Preconditioning of Rabbit Mesenchymal Stem Cells with Low Intensity Ultrasound to Induce Chondrogenesis in Vivo

INTRODUCTION For tissue regeneration, in vivo implantation of undifferentiated multipotent MSCs may pose a high risk of unforeseen events, such as heterotopic tissue formation. Therefore, regulatory signals of in vitro differentiation of MSCs are of importance and have been extensively studied for the induction of proper lineage commitment. In order to use MSCs to repair articular cartilage, it...

Induction of Adhesion-dependent Signals Using Low-intensity Ultrasound

In multicellular organisms, cell behavior is dictated by interactions with the extracellular matrix. Consequences of matrix-engagement range from regulation of cell migration and proliferation, to secretion and even differentiation. The signals underlying each of these complex processes arise from the molecular interactions of extracellular matrix receptors on the surface of the cell. Integrins...